Inactivation of 14-3-3sigma influences telomere behavior and ionizing radiation-induced chromosomal instability.

ABSTRACT: Telomeres are complexes of repetitive DNA sequences and proteins constituting the ends of linear eukaryotic chromosomes. While these structures are thought to be associated with the nuclear matrix, they appear to be released from this matrix at the time when the cells exit from G(2) and enter M phase. Checkpoints maintain the order and fidelity of the eukaryotic cell cycle, and defects in checkpoints contribute to genetic instability and cancer. The 14-3-3sigma gene has been reported to be a checkpoint control gene, since it promotes G(2) arrest following DNA damage. Here we demonstrate that inactivation of this gene influences genome integrity and cell survival. Analyses of chromosomes at metaphase showed frequent losses of telomeric repeat sequences, enhanced frequencies of chromosome en

SUBMITTER: Dhar S 

PROVIDER: S-EPMC86360 | biostudies-literature | 2000 Oct

REPOSITORIES: biostudies-literature