A high-throughput pipeline for scalable kit-free RNA extraction.
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ABSTRACT:
SUBMITTER: Han P
PROVIDER: S-EPMC8636496 | biostudies-literature | 2021 Dec
REPOSITORIES: biostudies-literature
Publications
A high-throughput pipeline for scalable kit-free RNA extraction.
Han Ping P Go Maybelle K MK Chow Jeng Yeong JY Xue Bo B Lim Yan Ping YP Crone Michael A MA Storch Marko M Freemont Paul S PS Yew Wen Shan WS
Scientific reports 20211201 1
An overreliance on commercial, kit-based RNA extraction in the molecular diagnoses of infectious disease presents a challenge in the event of supply chain disruptions and can potentially hinder testing capacity in times of need. In this study, we adapted a well-established, robust TRIzol-based RNA extraction protocol into a high-throughput format through miniaturization and automation. The workflow was validated by RT-qPCR assay for SARS-CoV-2 detection to illustrate its scalability without inte ...[more]
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