Characterization of a G-quadruplex from hepatitis B virus and its stabilization by binding TMPyP4, BRACO19 and PhenDC3.

ABSTRACT: Specific guanine rich nucleic acid sequences can form non-canonical structures, like the four stranded G-quadruplex (GQ). We studied the GQ-forming sequence (named HepB) found in the genome of the hepatitis B virus. Fluorescence-, infrared- and CD-spectroscopy were used. HepB shows a hybrid form in presence of K⁺, but Na⁺, Li⁺, and Rb⁺ induce parallel structure. Higher concentrations of metal ions increase the unfolding temperature, which was explained by a short thermodynamic calculation. Temperature stability of the GQ structure was determined for all these ions. Na⁺ has stronger stabilizing effect on HepB than K⁺, which is highly unusual. The transition temperatures were 56.6, 53.8, 58.5 and 54.4 °C for Na⁺, K<

SUBMITTER: Molnar OR 

PROVIDER: S-EPMC8636512 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature