Cross-species analysis of viral nucleic acid interacting proteins identifies TAOKs as innate immune regulators.

ABSTRACT: The cell intrinsic antiviral response of multicellular organisms developed over millions of years and critically relies on the ability to sense and eliminate viral nucleic acids. Here we use an affinity proteomics approach in evolutionary distant species (human, mouse and fly) to identify proteins that are conserved in their ability to associate with diverse viral nucleic acids. This approach shows a core of orthologous proteins targeting viral genetic material and species-specific interactions. Functional characterization of the influence of 181 candidates on replication of 6 distinct viruses in human cells and flies identifies 128 nucleic acid binding proteins with an impact on virus growth. We identify the family of TAO kinases (TAOK1, -2 and -3) as dsRNA-interacting antiviral proteins

SUBMITTER: Pennemann FL 

PROVIDER: S-EPMC8636641 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature