Potential of polylactic-co-glycolic acid (PLGA) for delivery Jembrana disease DNA vaccine Model (pEGFP-C1-tat).

ABSTRACT:

Background

The development of a vaccine for Jembrana disease is needed to prevent losses in Indonesia's Bali cattle industry. A DNA vaccine model (pEGFP-C1-tat) that requires a functional delivery system will be developed. Polylactic-co-glycolic acid (PLGA) may have potential as a delivery system for the vaccine model.

Objectives

This study aims to evaluate the in vitro potential of PLGA as a delivery system for pEGFP-C1-tat.

Methods

Consensus and codon optimization for the tat gene was completed using a bioinformatic method, and the product was inserted into a pEGFP-C1 vector. Cloning of the pEGFP-C1-tat was successfully performed, and polymerase chain reaction (PCR) and restriction analysis confirmed DNA isolation. PLGA-pEGFP-C1-tat solutions were prepared fo

SUBMITTER: Unsunnidhal L 

PROVIDER: S-EPMC8636661 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature