Morphological Response in Cancer Spheroids for Screening Photodynamic Therapy Parameters.

ABSTRACT: Photodynamic therapy (PDT) is a treatment which uses light-activated compounds to produce reactive oxygen species, leading to membrane damage and cell death. Multicellular cancer spheroids are a preferable alternative for PDT evaluation in comparison to monolayer cell cultures due to their ability to better mimic in vivo avascular tumour characteristics such as hypoxia and cell-cell interactions, low cost, and ease of production. However, inconsistent growth kinetics and drug responsiveness causes poor experimental reproducibility and limits their usefulness. Herein, we used image analysis to establish a link between human melanoma C8161 spheroid morphology and drug responsiveness. Spheroids were pre-selected based on sphericity, area, and diameter, reducing variation in experimenta

SUBMITTER: Aguilar Cosme JR 

PROVIDER: S-EPMC8637197 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature