Analyzing gut microbiota composition in individual Anopheles mosquitoes after experimental treatment.

ABSTRACT: The microbiota of Anopheles mosquitoes influences malaria transmission. Antibiotics ingested during a blood meal impact the mosquito microbiome and malaria transmission, with substantial differences between drugs. Here, we assessed if amoxicillin affects the gut mosquito microbiota. We collected Anopheles larvae in Burkina Faso, kept them in semi-field conditions, and offered a blood meal to adult females. We tested the impact of blood supplementation with two alternative amoxicillin preparations on microbiota composition, determined by high-throughput sequencing in individual gut samples. Our analysis detected four major genera, Elizabethkingia, Wigglesworthia, Asaia, and Serratia. The antibiotic treatment significantly affected overall mic

SUBMITTER: Fofana A 

PROVIDER: S-EPMC8637483 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature