Structure, Function and Regulation of a Second Pyruvate Kinase Isozyme in Pseudomonas aeruginosa.

ABSTRACT: Pseudomonas aeruginosa (PA) depends on the Entner-Doudoroff pathway (EDP) for glycolysis. The main enzymatic regulator in the lower half of the EDP is pyruvate kinase. PA contains genes that encode two isoforms of pyruvate kinase, denoted PykA_PA and PykF_PA. In other well-characterized organisms containing two pyruvate kinase isoforms (such as Escherichia coli) each isozyme is differentially regulated. The structure, function and regulation of PykA_PA has been previously characterized in detail, so in this work, we set out to assess the biochemical and structural properties of the PykF_PA isozyme. We show that pykF _PA expression is induced in the presence of the diureide, allantoin. In spite of their relatively low am

SUBMITTER: Abdelhamid Y 

PROVIDER: S-EPMC8637920 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature