Validated Quantitative ¹H NMR Method for Simultaneous Quantification of Indole Alkaloids in Uncaria rhynchophylla.

ABSTRACT: Uncariae Ramulus Cum Uncis, known as "Gou-Teng" in Chinese, is derived mainly from the dried hook-bearing stems of Uncaria rhynchophylla. Quantitative determination of monoterpenoid indole alkaloids is critical for controlling its quality. In the present study, a rapid, accurate, and precise method was developed for the simultaneous quantitation of four characteristic components, namely, rhynchophylline (1), isorhynchophylline (2), corynoxeine (3), and isocorynoxeine (4), through ¹H NMR spectrometry techniques. This method was performed on a 600 MHz NMR spectrometer with optimized acquisition parameters for performing quantitative experiments within 14 min. The highly deshielded signal of NH was at δ_H 10-11 in the aprotic solvent DMS

SUBMITTER: Yin T 

PROVIDER: S-EPMC8638010 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature