Methane Generation from Anthracite by Fungi and Methanogen Mixed Flora Enriched from Produced Water Associated with the Qinshui Basin in China.

ABSTRACT: Biogenic coalbed methane (CBM) is generally believed to be formed by anaerobic bacteria and methanogens, while a few studies took fungi into account. Here, the microflora consisting of fungi and methanogens was enriched from the produced water associated with the Qinshui Basin using anthracite as the only carbon source. The maximum methane yield of 231 μmol/g coal was obtained after 22 days of cultivation under the optimum temperature of 35 °C, pH of 8, salinity of 0-2%, particle size of 0.075-0.150 mm, and the solid-liquid ratio of 1:30. It could remain active even after exposure to air for 24 h. Miseq results showed that the archaea were mainly composed of Methanocella, a hydrogenotrophic methanogen, followed by acetoclastic methanogen Methanosaeta and Methanosarcina

SUBMITTER: Han Q 

PROVIDER: S-EPMC8638023 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature