Heterozygous Mutation of Vegfr3 Reduces Renal Lymphatics without Renal Dysfunction.

ABSTRACT:

Background

Lymphatic abnormalities are observed in several types of kidney disease, but the relationship between the renal lymphatic system and renal function is unclear. The discovery of lymphatic-specific proteins, advances in microscopy, and available genetic mouse models provide the tools to help elucidate the role of renal lymphatics in physiology and disease.

Methods

We utilized a mouse model containing a missense mutation in Vegfr3 (dubbed Chy) that abrogates its kinase ability. Vegfr3^Chy/+ mice were examined for developmental abnormalities and kidney-specific outcomes. Control and Vegfr3^Chy/+ mice were subjected to cisplatin-mediated injury. We characterized renal lymphatics using tissue-clearing, light-sheet microsco

SUBMITTER: Liu H 

PROVIDER: S-EPMC8638391 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature