Development of a mouse model of ascending infection and preterm birth.

ABSTRACT:

Background

Microbial invasion of the intraamniotic cavity and intraamniotic inflammation are factors associated with spontaneous preterm birth. Understanding the route and kinetics of infection, sites of colonization, and mechanisms of host inflammatory response is critical to reducing preterm birth risk.

Objectives

This study developed an animal model of ascending infection and preterm birth with live bacteria (E. coli) in pregnant CD-1 mice with the goal of better understanding the process of microbial invasion of the intraamniotic cavity and intraamniotic inflammation.

Study design

Multiple experiments were conducted in this study. To determine the dose of E. coli required to induce preterm birth, CD-1 mice were injected vaginally with four different doses of E. co

SUBMITTER: Spencer NR 

PROVIDER: S-EPMC8638907 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature