Late adolescence mortality in mice with brain-specific deletion of the volume-regulated anion channel subunit LRRC8A.

ABSTRACT: The leucine-rich repeat-containing family 8 member A (LRRC8A) is an essential subunit of the volume-regulated anion channel (VRAC). VRAC is critical for cell volume control, but its broader physiological functions remain under investigation. Recent studies in the field indicate that Lrrc8a disruption in the brain astrocytes reduces neuronal excitability, impairs synaptic plasticity and memory, and protects against cerebral ischemia. In the present work, we generated brain-wide conditional LRRC8A knockout mice (LRRC8A bKO) using Nestin^Cre -driven Lrrc8a^flox/flox excision in neurons, astrocytes, and oligodendroglia. LRRC8A bKO animals were born close to the expected Mendelian ratio and developed without overt histological abnormalities, but, surprisingly, all died betwe

SUBMITTER: Wilson CS 

PROVIDER: S-EPMC8639177 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature