LEDitSHAKE: a lighting system to optimize the secondary metabolite content of plant cell suspension cultures.

ABSTRACT: Plant secondary metabolites are widely used in the food, cosmetic and pharmaceutical industries. They can be extracted from sterile grown plant cell suspension cultures, but yields and quality strongly depend on the cultivation environment, including optimal illumination. Current shaking incubators do not allow different light wavelengths, intensities and photoperiods to be tested in parallel. We therefore developed LEDitSHAKE, a system for multiplexed customized illumination within a single shaking incubator. We used 3D printing to integrate light-emitting diode assemblies into flask housings, allowing 12 different lighting conditions (spectrum, intensity and photoperiod) to be tested simultaneously. We did a proof of principle of LEDitSHAKE using the system to optimize anthocyanin produc

SUBMITTER: Beuel AK 

PROVIDER: S-EPMC8639678 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature