Influence of Primary Structure on Fragmentation of Native-Like Proteins by Ultraviolet Photodissociation.

ABSTRACT: Analysis of native-like protein structures in the gas phase via native mass spectrometry and auxiliary techniques has become a powerful tool for structural biology applications. In combination with ultraviolet photodissociation (UVPD), native top-down mass spectrometry informs backbone flexibility, topology, hydrogen bonding networks, and conformational changes in protein structure. Although it is known that the primary structure affects dissociation of peptides and proteins in the gas phase, its effect on the types and locations of backbone cleavages promoted by UVPD and concomitant influence on structural characterization of native-like proteins is not well understood. Here, trends in the fragmentation of native-like proteins were evaluated by tracking the propensity of 10 fragment types

SUBMITTER: Macias LA 

PROVIDER: S-EPMC8639798 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature