Efficacy and immunogenicity of different BCG doses in BALB/c and CB6F1 mice when challenged with H37Rv or Beijing HN878.

ABSTRACT: Two strains of mice (BALB/c and CB6F1) were vaccinated with a range of Bacille Calmette-Guérin (BCG) Danish doses from 3 × 10⁵ to 30 CFU/mouse, followed by aerosol infection with Mtb (H37Rv or West-Beijing HN878 strain). The results indicated that both strains of mice when infected with HN878 exhibited significant protection in their lungs with BCG doses at 3 × 10⁵-3000 CFU (BALB/c) and 3 × 10⁵-300 CFU (CB6F1). Whereas, a significant protection was seen in both strains of mice with BCG doses at 3 × 10⁵-300 CFU when infected with H37Rv. A significant increase in the frequencies of BCG-specific IFNγ⁺ IL2⁺ TNFα⁺ CD4 T cells in the BCG doses at 3 × 10⁵-3000 CFU (BALB/c) and 3 × 10⁵-300 CFU (CB6F1

SUBMITTER: Khatri B 

PROVIDER: S-EPMC8639814 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature