Light-mediated discovery of surfaceome nanoscale organization and intercellular receptor interaction networks.

ABSTRACT: The molecular nanoscale organization of the surfaceome is a fundamental regulator of cellular signaling in health and disease. Technologies for mapping the spatial relationships of cell surface receptors and their extracellular signaling synapses would unlock theranostic opportunities to target protein communities and the possibility to engineer extracellular signaling. Here, we develop an optoproteomic technology termed LUX-MS that enables the targeted elucidation of acute protein interactions on and in between living cells using light-controlled singlet oxygen generators (SOG). By using SOG-coupled antibodies, small molecule drugs, biologics and intact viral particles, we demonstrate the ability of LUX-MS to decode ligand receptor interactions across organisms and to discover surfaceome

SUBMITTER: Muller M 

PROVIDER: S-EPMC8639842 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature