GlomSpheres as a 3D co-culture spheroid model of the kidney glomerulus for rapid drug-screening.

ABSTRACT: The glomerulus is the filtration unit of the kidney. Injury to any component of this specialised structure leads to impaired filtration and eventually fibrosis and chronic kidney disease. Current two and three dimensional (2D and 3D) models that attempt to recreate structure and interplay between glomerular cells are imperfect. Most 2D models are simplistic and unrepresentative, and 3D organoid approaches are currently difficult to reproduce at scale and do not fit well with current industrial drug-screening approaches. Here we report a rapidly generated and highly reproducible 3D co-culture spheroid model (GlomSpheres), better demonstrating the specialised physical and molecular structure of a glomerulus. Co-cultured using a magnetic spheroid formation approach, conditionally immortalised

SUBMITTER: Tuffin J 

PROVIDER: S-EPMC8640035 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature