A Pulmonary Vascular Model From Endothelialized Whole Organ Scaffolds.

ABSTRACT: The development of an in vitro system for the study of lung vascular disease is critical to understanding human pathologies. Conventional culture systems fail to fully recapitulate native microenvironmental conditions and are typically limited in their ability to represent human pathophysiology for the study of disease and drug mechanisms. Whole organ decellularization provides a means to developing a construct that recapitulates structural, mechanical, and biological features of a complete vascular structure. Here, we developed a culture protocol to improve endothelial cell coverage in whole lung scaffolds and used single-cell RNA-sequencing analysis to explore the impact of decellularized whole lung scaffolds on endothelial phenotypes and functions in a biomimetic bioreactor syste

SUBMITTER: Yuan Y 

PROVIDER: S-EPMC8640093 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature