Murine cerebral organoids develop network of functional neurons and hippocampal brain region identity.

ABSTRACT: Brain organoids are in vitro three-dimensional (3D) self-organized neural structures, which can enable disease modeling and drug screening. However, their use for standardized large-scale drug screening studies is limited by their high batch-to-batch variability, long differentiation time (10-20 weeks), and high production costs. This is particularly relevant when brain organoids are obtained from human induced pluripotent stem cells (iPSCs). Here, we developed, for the first time, a highly standardized, reproducible, and fast (5 weeks) murine brain organoid model starting from embryonic neural stem cells. We obtained brain organoids, which progressively differentiated and self-organized into 3D networks of functional neurons with dorsal forebrain phenotype. Furthermore, by adding t

SUBMITTER: Ciarpella F 

PROVIDER: S-EPMC8640475 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature