Three-dimensional virtual histology of the human hippocampus based on phase-contrast computed tomography.

ABSTRACT: We have studied the three-dimensional (3D) cytoarchitecture of the human hippocampus in neuropathologically healthy and Alzheimer's disease (AD) individuals, based on phase-contrast X-ray computed tomography of postmortem human tissue punch biopsies. In view of recent findings suggesting a nuclear origin of AD, we target in particular the nuclear structure of the dentate gyrus (DG) granule cells. Tissue samples of 20 individuals were scanned and evaluated using a highly automated approach of measurement and analysis, combining multiscale recordings, optimized phase retrieval, segmentation by machine learning, representation of structural properties in a feature space, and classification based on the theory of optimal transport. Accordingly, we find that the prototypical transformation betw

SUBMITTER: Eckermann M 

PROVIDER: S-EPMC8640721 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature