Identification of transcription factors and construction of a novel miRNA regulatory network in primary osteoarthritis by integrated analysis.

ABSTRACT:

Backgrounds

As osteoarthritis (OA) disease-modifying therapies are not available, novel therapeutic targets need to be discovered and prioritized. Here, we aim to identify miRNA signatures in patients to fully elucidate regulatory mechanism of OA pathogenesis and advance in basic understanding of the genetic etiology of OA.

Methods

Six participants (3 OA and 3 controls) were recruited and serum samples were assayed through RNA sequencing (RNA-seq). And, RNA-seq dataset was analysed to identify genes, pathways and regulatory networks dysregulated in OA. The overlapped differentially expressed microRNAs (DEMs) were further screened in combination with the microarray dataset GSE143514. The expression levels of candidate miRNAs were further validated by quantitative real-time PC

SUBMITTER: Jiang Y 

PROVIDER: S-EPMC8641180 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature