An Integrated Epigenomic and Transcriptomic Map of Mouse and Human αβ T Cell Development.

ABSTRACT: αβ lineage T cells, most of which are CD4⁺ or CD8⁺ and recognize MHC I- or MHC II-presented antigens, are essential for immune responses and develop from CD4⁺CD8⁺ thymocytes. The absence of in vitro models and the heterogeneity of αβ thymocytes have hampered analyses of their intrathymic differentiation. Here, combining single-cell RNA and ATAC (chromatin accessibility) sequencing, we identified mouse and human αβ thymocyte developmental trajectories. We demonstrated asymmetric emergence of CD4⁺ and CD8⁺ lineages, matched differentiation programs of agonist-signaled cells to their MHC specificity, and identified correspondences between mouse and human transcriptomic and epigenomic patterns. Through computational analysis of

SUBMITTER: Chopp LB 

PROVIDER: S-EPMC8641659 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature