Methamphetamine leads to the alterations of microRNA profiles in the nucleus accumbens of rats.

ABSTRACT:

Context

MicroRNA (miRNA) is an important regulator of gene expression. Methamphetamine (METH) induces a variety of alterations in different systems by affecting gene expression, but the effects of METH on miRNA profiles need to be elucidated.

Objectives

This study develops a rat model of METH addiction, and analyzes the expression profile alterations of miRNA in nucleus accumbens (NAc) of the METH-addicted rats.

Materials and methods

Sprague-Dawley rats were administered 10 mg/kg METH or vehicle twice a day for 4 weeks. The addictive behaviour of rats was estimated by CPP test. The pathological changes of brain tissues were then observed by HE and Glee silver staining. The miRNA profile analysis of the NAc of the rats was performed using an Illumina HiSeq™ 2500 sequen

SUBMITTER: Yang J 

PROVIDER: S-EPMC8641683 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature