Discriminative feature of cells characterizes cell populations of interest by a small subset of genes.

ABSTRACT: Organisms are composed of various cell types with specific states. To obtain a comprehensive understanding of the functions of organs and tissues, cell types have been classified and defined by identifying specific marker genes. Statistical tests are critical for identifying marker genes, which often involve evaluating differences in the mean expression levels of genes. Differentially expressed gene (DEG)-based analysis has been the most frequently used method of this kind. However, in association with increases in sample size such as in single-cell analysis, DEG-based analysis has faced difficulties associated with the inflation of P-values. Here, we propose the concept of discriminative feature of cells (DFC), an alternative to using DEG-based approaches. We implemented DFC using logisti

SUBMITTER: Fujii T 

PROVIDER: S-EPMC8641884 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature