A point-of-care lateral flow assay for neutralising antibodies against SARS-CoV-2.

ABSTRACT:

Background

As vaccines against SARS-CoV-2 are now being rolled out, a better understanding of immunity to the virus, whether from infection, or passive or active immunisation, and the durability of this protection is required. This will benefit from the ability to measure antibody-based protection to SARS-CoV-2, ideally with rapid turnaround and without the need for laboratory-based testing.

Methods

We have developed a lateral flow POC test that can measure levels of RBD-ACE2 neutralising antibody (NAb) from whole blood, with a result that can be determined by eye or quantitatively on a small instrument. We compared our lateral flow test with the gold-standard microneutralisation assay, using samples from convalescent and vaccinated donors, as well as immunised macaques.

SUBMITTER: Fulford TS 

PROVIDER: S-EPMC8641961 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature