Hsp70 Promotes SUMO of HIF-1α and Promotes Lung Cancer Invasion and Metastasis.

ABSTRACT:

Objective

This study aims to investigate the effect of heat shock protein-70 (Hsp70) on epithelial-mesenchymal transition (EMT) of lung cancer cells under heat stimulation and to explore its possible molecular mechanism.

Methods

qRT-PCR and immunohistochemistry assay were used to detect the expression of Hsp70 in lung cancer tissues and adjacent tissues. EdU assay was used to detect the cell activity. The effect of Hsp70 on the migration and invasion of A549 and NCI-H446 cells was detected by the wound-healing assay and Transwell assay. A tumor transplantation animal model was established to detect the effect of overexpression of Hsp70 on proliferation and metastasis of lung cancer cells. Western blot assay was used to detect the effect of thermal stimulation and overexpress

SUBMITTER: Ling X 

PROVIDER: S-EPMC8642011 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature