Isolation of multipotent progenitor cells from pleura and pericardium for tracheal tissue engineering purposes.

ABSTRACT: Tissue engineering (TE) of long tracheal segments is conceptually appealing for patients with inoperable tracheal pathology. In tracheal TE, stem cells isolated from bone marrow or adipose tissue have been employed, but the ideal cell source has yet to be determined. When considering the origin of stem cells, cells isolated from a source embryonically related to the trachea may be more similar. In this study, we investigated the feasibility of isolating progenitor cells from pleura and pericard as an alternative cells source for tracheal tissue engineering. Porcine progenitor cells were isolated from pleura, pericard, trachea and adipose tissue and expanded in culture. Isolated cells were characterized by PCR, RNA sequencing, differentiation assays and cell survival assays and were compare

SUBMITTER: de Wit R 

PROVIDER: S-EPMC8642678 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature