Gene regulatory networks controlling temporal patterning, neurogenesis, and cell-fate specification in mammalian retina.

ABSTRACT: Gene regulatory networks (GRNs), consisting of transcription factors and their target sites, control neurogenesis and cell-fate specification in the developing central nervous system. In this study, we use integrated single-cell RNA and single-cell ATAC sequencing (scATAC-seq) analysis in developing mouse and human retina to identify multiple interconnected, evolutionarily conserved GRNs composed of cell-type-specific transcription factors that both activate genes within their own network and inhibit genes in other networks. These GRNs control temporal patterning in primary progenitors, regulate transition from primary to neurogenic progenitors, and drive specification of each major retinal cell type. We confirm that NFI transcription factors selectively activate expression of genes promot

SUBMITTER: Lyu P 

PROVIDER: S-EPMC8642835 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature