Metabarcoding in two isolated populations of wild roe deer (Capreolus capreolus) reveals variation in gastrointestinal nematode community composition between regions and among age classes.

ABSTRACT:

Background

Gastrointestinal nematodes are ubiquitous for both domestic and wild ungulates and have varying consequences for health and fitness. They exist as complex communities of multiple co-infecting species, and we have a limited understanding of how these communities vary in different hosts, regions and circumstances or of how this affects their impacts.

Methods

We have undertaken ITS2 rDNA nemabiome metabarcoding with next-generation sequencing on populations of nematode larvae isolated from 149 fecal samples of roe deer of different sex and age classes in the two isolated populations of Chizé and Trois Fontaines in France not co-grazing with any domestic ungulate species.

Results

We identified 100 amplified sequence variants (ASVs) that were assigned to 14 gast

SUBMITTER: Beaumelle C 

PROVIDER: S-EPMC8642965 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature