ASS1 and ASL suppress growth in clear cell renal cell carcinoma via altered nitrogen metabolism.

ABSTRACT:

Background

Kidney cancer is a common adult malignancy in the USA. Clear cell renal cell carcinoma (ccRCC), the predominant subtype of kidney cancer, is characterized by widespread metabolic changes. Urea metabolism is one such altered pathway in ccRCC. The aim of this study was to elucidate the contributions of urea cycle enzymes, argininosuccinate synthase 1 (ASS1), and argininosuccinate lyase (ASL) towards ccRCC progression.

Methods

We employed a combination of computational, genetic, and metabolomic tools along with in vivo animal models to establish a tumor-suppressive role for ASS1 and ASL in ccRCC.

Results

We show that the mRNA and protein expression of urea cycle enzymes ASS1 and ASL are reduced in ccRCC tumors when compared to the normal kidney. Furthermore, t

SUBMITTER: Khare S 

PROVIDER: S-EPMC8642968 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature