Semi-stable Production of Bovine IL-4 and GM-CSF in The Mammalian Episomal Expression System.

ABSTRACT:

Introduction

Granulocyte-macrophage colony stimulating factor (GM-CSF) and interleukin-4 (IL-4) are cytokines widely used in ex vivo monocyte differentiation experiments, vaccine formulations and disease treatment. The aim of this study was to produce recombinant bovine GM-CSF and IL-4 in an episomal expression system that conserves the postransductional modification of the native proteins and to use the products to differentiate bovine monocytes into dendritic cells.

Material and methods

The recombinant proteins rGM-CSF and rIL-4 were expressed in PEAKrapid CRL-2828 human kidney cells, ATCC CRL-2828. The functional activity of the recombinant cytokines was monitored by registering morphological changes in bovine monocytes and assessing the expression of CD14 upon inc

SUBMITTER: Blanco FC 

PROVIDER: S-EPMC8643090 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature