Role of PD-L1 in licensing immunoregulatory function of dental pulp mesenchymal stem cells.

ABSTRACT:

Background

Dental pulp stem cells (DPSCs) are low immunogenic and hold immunomodulatory properties that, along with their well-established multi-potency, might enhance their potential application in autoimmune and inflammatory diseases. The present study focused on the ability of DPSCs to modulate the inflammatory microenvironment through PD1/PD-L1 pathway.

Methods

Inflammatory microenvironment was created in vitro by the activation of T cells isolated from healthy donors and rheumatoid arthritis (RA) patients with anti-CD3 and anti-CD28 antibodies. Direct and indirect co-cultures between DPSCs and PBMCs were carried out to evaluate the activation of immunomodulatory checkpoints in DPSCs and the inflammatory pattern in PBMCs.

Results

Our data suggest that the inflamma

SUBMITTER: Di Tinco R 

PROVIDER: S-EPMC8643194 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature