MRNA and long non-coding RNA expression profiling of human periodontal ligament cells under tension loading.

ABSTRACT:

Objective

This study explored the expression profiles of messenger RNAs (mRNAs) and long non-coding RNAs (lncRNAs) in human periodontal ligament (PDL) cells subjected to tensile loading.

Methods

PDL cells were isolated from the teeth of five healthy individuals, cultured and then exposed to tensile loading. RNA sequencing was performed to explore the mRNA and lncRNA expression profiles with or without tensile loading. Differential expression, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were conducted to reveal enriched biological functions and signal transduction pathways. Quantitative polymerase chain reaction (qPCR) was performed to validate the expression of specific mRNAs and lncRNAs associated with the enriched pathways.

SUBMITTER: Lin Y 

PROVIDER: S-EPMC8643418 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature