Blood-brain barrier alterations in human brain tumors revealed by genome-wide transcriptomic profiling.

ABSTRACT:

Background

Brain tumors, whether primary or secondary, have limited therapeutic options despite advances in understanding driver gene mutations and heterogeneity within tumor cells. The cellular and molecular composition of brain tumor stroma, an important modifier of tumor growth, has been less investigated to date. Only few studies have focused on the vasculature of human brain tumors despite the fact that the blood-brain barrier (BBB) represents the major obstacle for efficient drug delivery.

Methods

In this study, we employed RNA sequencing to characterize transcriptional alterations of endothelial cells (EC) isolated from primary and secondary human brain tumors. We used an immunoprecipitation approach to enrich for EC from normal brain, glioblastoma (GBM), and lung can

SUBMITTER: Schaffenrath J 

PROVIDER: S-EPMC8643445 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature