Experimental design of preclinical experiments: number of PDX lines vs subsampling within PDX lines.

ABSTRACT:

Background

Appropriately designed preclinical patient-derived xenograft (PDX) experiments are important to accurately inform human clinical trials. There is little experimental design guidance regarding choosing the number of PDX lines to study, and the number of mice within each PDX line.

Methods

Retrospective data from IDH-wildtype glioblastoma preclinical experiments evaluating a uniform regimen of fractionated radiation (RT), temozolomide (TMZ) chemotherapy, and concurrent RT/TMZ across 27 PDX lines were used to evaluate experimental designs and empirically estimate statistical power for ANOVA and Cox regression.

Results

Increasing the number of PDX lines resulted in more precise and reproducible estimates of effect size. To achieve 80% statistical power using ANO

SUBMITTER: Eckel-Passow JE 

PROVIDER: S-EPMC8643474 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature