Novel Aspects in Inhibiting Pancreatic Lipase with Potential New Compound from Nutmeg in Connection with Obesity - In Vitro, In Silico, In Vivo and Ex Vivo Studies.

ABSTRACT: Obesity is now considered a "new world syndrome" because it is affecting developed and developing nations in all classes of people. Pancreatic lipase (PL) is the key enzyme for absorption of dietary lipids. Excessive consumption of cafeteria foods leads to obesity. Nutmeg is being used as a spice and it has many medicinal values. We aimed to see whether nutmeg could be used as a potential source of new lipase inhibitors and to explore its molecular mechanism in obesity. An in vitro study, which was performed to check its anti-lipase activity, showed a potent lipase inhibition (66.24%). A liquid chromatography-mass spectrometry (LC-MS) analysis was carried out to identify new compounds, and tetrahydrofuran (THF) with MW 536 at RT 15.2 min was isolated. A molecular docking study, that was ca

SUBMITTER: Yakaiah V 

PROVIDER: S-EPMC8643554 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature