A new RNA-DNA interaction required for integration of group II intron retrotransposons into DNA targets.

ABSTRACT: Mobile group II introns are site-specific retrotransposable elements abundant in bacterial and organellar genomes. They are composed of a large and highly structured ribozyme and an intron-encoded reverse transcriptase that binds tightly to its intron to yield a ribonucleoprotein (RNP) particle. During the first stage of the mobility pathway, the intron RNA catalyses its own insertion directly into the DNA target site. Recognition of the proper target rests primarily on multiple base-pairing interactions between the intron RNA and the target DNA, while the protein makes contacts with only a few target positions by yet-unidentified mechanisms. Using a combination of comparative sequence analyses and in vivo mobility assays we demonstrate the existence of a new base-pairing interaction named

SUBMITTER: Monachello D 

PROVIDER: S-EPMC8643678 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature