Cellular heterogeneity in DNA alkylation repair increases population genetic plasticity.

ABSTRACT: DNA repair mechanisms fulfil a dual role, as they are essential for cell survival and genome maintenance. Here, we studied how cells regulate the interplay between DNA repair and mutation. We focused on the adaptive response that increases the resistance of Escherichia coli cells to DNA alkylation damage. Combination of single-molecule imaging and microfluidic-based single-cell microscopy showed that noise in the gene activation timing of the master regulator Ada is accurately propagated to generate a distinct subpopulation of cells in which all proteins of the adaptive response are essentially absent. Whereas genetic deletion of these proteins causes extreme sensitivity to alkylation stress, a temporary lack of expression is tolerated and increases genetic plasticity of the whole populati

SUBMITTER: Vincent MS 

PROVIDER: S-EPMC8643705 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature