Development and characterization of efficient xylose utilization strains of Zymomonas mobilis.

ABSTRACT:

Background

Efficient use of glucose and xylose is a key for the economic production of lignocellulosic biofuels and biochemicals, and different recombinant strains have been constructed for xylose utilization including those using Zymomonas mobilis as the host. However, the xylose utilization efficiency still needs to be improved. In this work, the strategy of combining metabolic engineering and adaptive laboratory evolution (ALE) was employed to develop recombinant Z. mobilis strains that can utilize xylose efficiently at high concentrations, and NGS-based genome resequencing and RNA-Seq transcriptomics were performed for strains evolved after serial transfers in different media to understand the impact of xylose and differences among strains with different xylose-utilization capa

SUBMITTER: Lou J 

PROVIDER: S-EPMC8645129 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature