Transcriptional Response of Multidrug-Resistant Klebsiella pneumoniae Clinical Isolates to Ciprofloxacin Stress.

ABSTRACT:

Background

The term "persisters" refers to a small bacterial population that persists during treatment with high antibiotic concentration or dose in the absence of genetic resistance. The present study was designed to investigate the transcriptional response in indigenous Klebsiella pneumoniae under the ciprofloxacin stress.

Methods

Isolation and identification of K. pneumoniae were carried out through standard microbiological protocols. The characterization of quinolone resistance was performed by estimating the quinolone susceptibility testing, MIC estimation, and detecting the QRDR and PMQR. Transcriptional response of the isolates to ciprofloxacin was determined using qPCR.

Results

Among 34 isolates, 23 (67%) were resistant to ciprofloxacin. Both QRD

SUBMITTER: Alvi RF 

PROVIDER: S-EPMC8645360 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature