Interferon inducible pseudouridine modification in human mRNA by quantitative nanopore profiling.

ABSTRACT: Pseudouridine (Ψ) is an abundant mRNA modification in mammalian transcriptome, but its functions have remained elusive due to the difficulty of transcriptome-wide mapping. We develop a nanopore native RNA sequencing method for quantitative Ψ prediction (NanoPsu) that utilizes native content training, machine learning modeling, and single-read linkage analysis. Biologically, we find interferon inducible Ψ modifications in interferon-stimulated gene transcripts which are consistent with a role of Ψ in enabling efficacy of mRNA vaccines.

SUBMITTER: Huang S 

PROVIDER: S-EPMC8646010 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature