Teaching an old dog new tricks: A lipid membrane-based electric immunosensor for real-time probing of the spike S₁ protein subunit from SARS-CoV-2.

ABSTRACT: Fast, cheap, and easy to implement point-of-care testing for various pathogens constituted a game changer in past years due to its potential for early disease diagnosis. Herein, we report on the proof-of-concept of a simple method enabling in vitro detection of a structural spike protein subunit from the SARS-CoV-2 (S₁ ) in aqueous samples. At the core of this discovery lies the well-known paradigm of monitoring the capacitive current across a reconstituted zwitterionic lipid membrane subjected to a periodic transmembrane potential, followed by the real-time spectral analysis enabling the extraction of the second harmonic of the capacitive current. Subsequent changes in the amplitude of this harmonic recorded during lipid membrane-S₁ interactions were correlated with

SUBMITTER: Asandei A 

PROVIDER: S-EPMC8646443 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature