Antibody-mediated procoagulant platelet formation in COVID-19 is AKT dependent.

ABSTRACT:

Background

Thromboembolic events are frequently reported in patients infected with the SARS-CoV-2. Recently, we observed that platelets from patients with severe COVID-19 infection express procoagulant phenotype. The molecular mechanisms that induce the generation of procoagulant platelets in COVID-19 patients are not completely understood.

Objectives

In this study, we investigated the role of AKT (also known as Protein Kinase B), which is the major downstream effector of PI3K (phosphoinositid-3-kinase) (PI3K/AKT) signaling pathway in platelets from patients with COVID-19.

Patients and methods

Platelets, Sera and IgG from COVID-19 patients who were admitted to the intensive care unit (ICU) were analyzed by flow cytometry as well as western blot and adhesion assays.

SUBMITTER: Pelzl L 

PROVIDER: S-EPMC8646637 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature