Helical filament structure of the DREP3 CIDE domain reveals a unified mechanism of CIDE-domain assembly.

ABSTRACT: The cell-death-inducing DFF45-like effector (CIDE) domain is a protein-interaction module comprising ∼80 amino acids and was initially identified in several apoptotic nucleases and their regulators. CIDE-domain-containing proteins were subsequently identified among proteins involved in lipid metabolism. Given the involvement of CIDE-domain-containing proteins in cell death and lipid homeostasis, their structure and function have been intensively studied. Here, the head-to-tail helical filament structure of the CIDE domain of DNA fragmentation factor-related protein 3 (DREP3) is presented. The helical filament structure was formed by opposing positively and negatively charged interfaces of the domain and was assembled depending on protein and salt concentrations. Although conserved filament

SUBMITTER: Lee SY 

PROVIDER: S-EPMC8647176 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature