A novel red fluorescence dopamine biosensor selectively detects dopamine in the presence of norepinephrine in vitro.

ABSTRACT: Dopamine (DA) and norepinephrine (NE) are pivotal neuromodulators that regulate a broad range of brain functions, often in concert. Despite their physiological importance, untangling the relationship between DA and NE in the fine control of output function is currently challenging, primarily due to a lack of techniques to allow the observation of spatiotemporal dynamics with sufficiently high selectivity. Although genetically encoded fluorescent biosensors have been developed to detect DA, their poor selectivity prevents distinguishing DA from NE. Here, we report the development of a red fluorescent genetically encoded GPCR (G protein-coupled receptor)-activation reporter for DA termed 'R-GenGAR-DA'. More specifically, a circular permutated red fluorescent protein (cpmApple) was replaced b

SUBMITTER: Nakamoto C 

PROVIDER: S-EPMC8647500 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature