Expression of a CO₂-permeable aquaporin enhances mesophyll conductance in the C₄ species Setaria viridis.

ABSTRACT: A fundamental limitation of photosynthetic carbon fixation is the availability of CO₂. In C₄ plants, primary carboxylation occurs in mesophyll cytosol, and little is known about the role of CO₂ diffusion in facilitating C₄ photosynthesis. We have examined the expression, localization, and functional role of selected plasma membrane intrinsic aquaporins (PIPs) from Setaria italica (foxtail millet) and discovered that SiPIP2;7 is CO₂-permeable. When ectopically expressed in mesophyll cells of Setaria viridis (green foxtail), SiPIP2;7 was localized to the plasma membrane and caused no marked changes in leaf biochemistry. Gas exchange and C¹⁸O¹⁶O discrimination measurements revealed that targeted expression of SiPIP2;7 enhanced the conductance to CO₂ diffusion from the intercellular airspace to the mesophyll cytosol. Our results demonstrate that mesophyll conductance limits C₄ photosynthesis at low pCO₂ and that SiPIP2;7 is a functional CO₂ permeable aquaporin that can improve CO₂ diffusion at the airspace/mesophyll interface and enhance C₄ photosynthesis.

SUBMITTER: Ermakova M 

PROVIDER: S-EPMC8648302 | biostudies-literature |

REPOSITORIES: biostudies-literature