Human-likeness of antibody biologics determined by back-translation and comparison with large antibody variable gene repertoires.

ABSTRACT: The antibody (Ab) germline gene rearrangement of variable (V), diversity (D), and joining (J) gene segments, as well as somatic hypermutation, give rise to the human Ab variable gene sequence repertoire. It is common to characterize single nucleotide frequencies of the variable region by alignment to species-specific wildtype germline genes. The increasing application of next-generation sequencing to immune repertoire studies has led to the compilation of increasing large adaptive immunome receptor repertoire datasets. We have developed a method that maps the sequence of a target Ab onto an immunome dataset of 326 million human Ab sequences. For this purpose, we created a position- and gene-specific scoring matrix (PGSSM) and its corresponding antibody similarity score. We characterized ou

SUBMITTER: Schmitz S 

PROVIDER: S-EPMC8648325 | biostudies-literature | 2020 Jan-Dec

REPOSITORIES: biostudies-literature