Dataset Information

Medical imaging of pulmonary disease in SARS-CoV-2-exposed non-human primates.

ABSTRACT: Chest X-ray (CXR), computed tomography (CT), and positron emission tomography-computed tomography (PET-CT) are noninvasive imaging techniques widely used in human and veterinary pulmonary research and medicine. These techniques have recently been applied in studies of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-exposed non-human primates (NHPs) to complement virological assessments with meaningful translational readouts of lung disease. Our review of the literature indicates that medical imaging of SARS-CoV-2-exposed NHPs enables high-resolution qualitative and quantitative characterization of disease otherwise clinically invisible and potentially provides user-independent and unbiased evaluation of medical countermeasures (MCMs). However, we also found high variability in image acquisition and analysis protocols among studies. These findings uncover an urgent need to improve standardization and ensure direct comparability across studies.

SUBMITTER: Stammes MA

PROVIDER: S-EPMC8648672 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature

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Publications

Medical imaging of pulmonary disease in SARS-CoV-2-exposed non-human primates.

Stammes Marieke A MA Lee Ji Hyun JH Meijer Lisette L Naninck Thibaut T Doyle-Meyers Lara A LA White Alexander G AG Borish H Jacob HJ Hartman Amy L AL Alvarez Xavier X Ganatra Shashank S Kaushal Deepak D Bohm Rudolf P RP le Grand Roger R Scanga Charles A CA Langermans Jan A M JAM Bontrop Ronald E RE Finch Courtney L CL Flynn JoAnne L JL Calcagno Claudia C Crozier Ian I Kuhn Jens H JH

Trends in molecular medicine 20211207 2

Chest X-ray (CXR), computed tomography (CT), and positron emission tomography-computed tomography (PET-CT) are noninvasive imaging techniques widely used in human and veterinary pulmonary research and medicine. These techniques have recently been applied in studies of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-exposed non-human primates (NHPs) to complement virological assessments with meaningful translational readouts of lung disease. Our review of the literature indicates tha ...[more]

PMID: 34955425

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Project description:Neurologic manifestations are among the most frequently reported complications of COVID-19. However, given the paucity of tissue samples and highly infectious nature of the etiologic agent of COVID-19, we have limited information to understand the neuropathogenesis of COVID-19. Therefore to better understand the impact of COVID-19 in brain, we employed mass-spectrometry-based proteomics using data-independent acquisition mode (DIA) to investigate cerebrospinal fluid (CSF) proteins collected from two different non-human primates, Rhesus Macaque and African Green Monkeys for the neurologic effects of the infection. These monkeys exhibited minimal to mild pulmonary pathology but moderate to severe CNS pathology. Our results indicate that CSF proteome changes after infection resolution correspond with bronchial virus abundance during early infection and revealed substantial differences between the infected NHPs and their age-matched uninfected controls, suggesting these differences could reflect altered secretion of CNS factors in response to SARS-CoV-2-induced neuropathology. We also observed the infected animals to exhibit much scattered data distributions as compared to the tightly clustered corresponding controls which suggest the heterogeneity of the CSF proteome change and the host response to the viral infection. In addition, dysregulated CSF proteins were preferentially enriched in functional pathways associated with progressive neurodegenerative disorders, hemostasis and innate immune responses that could influence neuroinflammatory responses following COVID-19. Mapping dysregulated proteins to the Human Brain Protein Atlas found that these proteins tended to be enriched in brain regions that exhibit more frequent injury following COVID-19. It therefore appears reasonable to speculate that such CSF protein changes could serve as signatures for neurologic injury, identify important regulatory pathways in this process, and potentially reveal therapeutic targets to prevent or attenuate the development of neurologic injuries following COVID-19.

Dataset Information

Medical imaging of pulmonary disease in SARS-CoV-2-exposed non-human primates.

Publications

Medical imaging of pulmonary disease in SARS-CoV-2-exposed non-human primates.

Similar Datasets

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