Acute Genetic Ablation of Cardiac Sodium/Calcium Exchange in Adult Mice: Implications for Cardiomyocyte Calcium Regulation, Cardioprotection, and Arrhythmia.

ABSTRACT: Background Sodium-calcium (Ca²⁺) exchanger isoform 1 (NCX1) is the dominant Ca²⁺ efflux mechanism in cardiomyocytes and is critical to maintaining Ca²⁺ homeostasis during excitation-contraction coupling. NCX1 activity has been implicated in the pathogenesis of cardiovascular diseases, but a lack of specific NCX1 blockers complicates experimental interpretation. Our aim was to develop a tamoxifen-inducible NCX1 knockout (KO) mouse to investigate compensatory adaptations of acute ablation of NCX1 on excitation-contraction coupling and intracellular Ca²⁺ regulation, and to examine whether acute KO of NCX1 confers resistance to triggered arrhythmia and ischemia/reperfusion injury. Methods and Results We used the α-myosin heavy chain promoter (Myh6)-M

SUBMITTER: Lotteau S 

PROVIDER: S-EPMC8649274 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature